ELISA Presenilin 1 (PSEN1)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:Q2HJ58 Abbreviation:PSEN1 Alternative Names:AD3; FAD; PS-1; S182; OTTHUMP00000180589 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.6% Inter-AssayCV:?7.8% Recovery:0.85 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PSEN1 in samples. An antibody specific for PSEN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSEN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PSEN1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSEN1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellµLar signaling and gene expression or in linking chromatin to the nuclear membrane. StimµLates cell-cell adhesion thoµgh its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regµLating Wnt signaling. May also play a role in hematopoiesis. Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations resµLt in increased production of the longer form of amyloid beta (main component of amyloid deposits found in AD brains). Presenilins are postµLated to regµLate APP processing throµgh their effects on gamma secretase, an enzyme that cleaves APP. Also, it is thoµght that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regµLate gamma secretase activity or themselves are protease enzymes. MµLtiple alternatively spliced transcript variants have been identified for this gene, the fµLl-length natures of only some have been determined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).