ELISA Bovine Nicotinate-nucleotide pyrophosphorylase (QPRT)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q3T063 Abbreviation:QPRT Alternative Names:QPRTase; nicotinate-nucleotide pyrophosphorylase (carboxylating) Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.9% Inter-AssayCV:?8.7% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate QPRT in samples. An antibody specific for QPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for QPRT is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QPRT bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Quinolinate is an intermediate in the de novo synthesis pathway of NAD from tryptophan and acts as a potent endogenous excitotoxin throµgh hyperstimµLation of N-methyl D-aspartate (NMDA) receptor in the neuron. Elevation of quinolinate levels in the brain has been postµLated to be involved in the pathogenesis of neurodegenerative disorders. Mammalian quinolinate phosphoribosyltransferase (QPRTase) is a key enzyme in catabolism of quinolinate.This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).