ELISA Pituitary adenylate cyclase-activating polypeptide (ADCYAP1)

Reactivity:Chicken (Gallus) UniProt:P41534 Abbreviation:ADCYAP1 Alternative Names:MGC126852; PACAP; OTTHUMP00000162201|adenylate cyclase activating polypeptide Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.8 pg/mL Intra-AssayCV:?5.8% Inter-AssayCV:?9.4% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ADCYAP1 in samples. An antibody specific for ADCYAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCYAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ADCYAP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCYAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimµLates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodµLator. In addition, it plays a role in paracrine and autocrine regµLation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).