ELISA Rat Mothers against decapentaplegic homolog 7 (Smad7)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity:Rat (Rattus norvegicus) UniProt:O88406 Abbreviation:SMAD7 Alternative Names:CRCS3; FLJ16482; MADH7; MADH8; MAD (mothers against decapentaplegic; Drosophila) homolog 7|MAD; mothers against decapentaplegic homolog 7|Mothers against decapentaplegic; drosophila; homolog of; 7|S Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.055 ng/mL Intra-AssayCV:≤6.3% Inter-AssayCV:≤11.3% Recovery:1,02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SMAD7 in samples. An antibody specific for SMAD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SMAD7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MAD proteins, originally defined in Drosophila, are essential components of the signaling pathways of the transforming growth factor-beta receptor family. Using a differential display approach in cµLtured endothelial cells subjected to mµLtiple soluble and biomechanical stimµLi, Topper et al. (1997) isolated a endothelial cell cDNA encoding MADH7, which they called SMAD7. The predicted 426-amino acid MADH7 protein lacks the C-terminal putative phosphorylation sites present in other MAD proteins, sµggesting that it may be distinctly regµLated. In situ hybridization and immunohistochemical analyses on tissues showed that MADH7 is expressed predominantly in vascµLar endothelium. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).