ELISA Sarcalumenin (SRL)

Reactivity:Chicken (Gallus) UniProt:Q90577 Abbreviation:SRL Alternative Names:N/A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.6% Inter-AssayCV:?9.3% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SRL in samples. An antibody specific for SRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SRL is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRL bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Sarcalumenin is a gene which encodes a 160-kD glycoprotein protein involved in calcium signaling.Muscle contraction is triggered by the release of calcium from the sarcoplasmic reticµLum, whereas muscle relaxation is achieved by rapid reuptake of calcium from the cytosol into the lumen of the sarcoplasmic reticµLum. Sequestration of calcium in the lumen of the sarcoplasmic reticµLum is also an essential step in the overall contraction-relaxation cycle of muscle cells. The rabbit sarcoplasmic reticµLum contains 2 immunochemically related glycoproteins with apparent molecµLar masses of 53 and 160 kD.The deduced 160-kD glycoprotein contains an N-terminal signal sequence and a 453-amino acid C-terminal sequence that together form a protein that is identical to the 53-kD glycoprotein. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).