ELISA Bovine Delta (14)-sterol reductase (TM7SF2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q8WMV1 Abbreviation:TM7SF2 Alternative Names:ANG1; DHCR14A; NET47; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.2% Inter-AssayCV:?10.8% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TM7SF2 in samples. An antibody specific for TM7SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM7SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TM7SF2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM7SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).