ELISA Bovine Nuclear transition protein 2 (TNP2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P26377 Abbreviation:TNP2 Alternative Names:MGC116783; MGC116785; TP2; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?7.6% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNP2 in samples. An antibody specific for TNP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TNP2 were not found in testicµLar RNA or testis cDNA libraries. Finding homologous sequences in the genome was greatly facilitated, however, by the finding that, in the boar and bµLl genomes, the genes for protamine-1 (PRM1) and Prm2 are clustered with Tnp2. Schluter et al. (1992) tested a genomic cosmid clone containing the genes for both protamines and found that the TNP2 gene was within a distance of 7.5 kb 3-prime of the PRM2 gene. The gene was transcribed in testicµLar RNA only at a level demonstrable by RT-PCR and RNase protection assay. In contrast to the TNP2 genes of mouse, rat, boar, and bµLl, the TNP2 gene lacks the nucleotide sequence GCCATCAC in its 3-prime UTR. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).