ELISA Tenascin-R (TNR)

Reactivity:Chicken (Gallus) UniProt:Q00546 Abbreviation:TNR Alternative Names:RP3-518E13.1; MGC149328; TN-R; OTTHUMP00000032802|tenascin R Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.5% Inter-AssayCV:?9.8% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNR in samples. An antibody specific for TNR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Tenascins are extracellµLar matrix glycoproteins. They are abundant in the extracellµLar matrix of developing vertebrate embryos and they reappear around healing wounds and in the stroma of some tumors.Tenascin-R is a tenascin gene.Tenascin-R is found in the developing and adµLt nervous system. Tenascin-R (TNR) is an extracellµLar matix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR (Erickson, 1993). The genes are expressed in distinct tissues at different times during embryonic development and are present in adµLt tissues. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).