ELISA CDC34 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regµLate cell proliferation throµgh ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjµgation and degradation of CREM isoform ICERIIgamma and ATF15 resµLting in abrogation of ICERIIgamma-and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regµLation of the cell cycle G2/M phase throµgh its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. Immunogen:cell division cycle 34 homolog(S. cerevisiae) Synonyms:UBCH3, UBE2R1 Observed MW:34 kDa Uniprot ID:P49427 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC Recommended dilution:WB: 1:500-1:2000; IHC: 1:20-1:200 Gene ID:997 Research Area:Epigenetics, Metabolism