ELISA CDK4 anti-

Quantity :100µL Clone Number: Aliases:Cdk 4 antibody; cdk4 antibody; CDK4 protein antibody; CDK4_ antibody; Cell division kinase 4 antibody; Cell division protein kinase 4 antibody; CMM 3 antibody; CMM3 antibody; Crk3 antibody; Cyclin dependent kinase 4 antibody; Cyclin-dependent kinase 4 antibody; Melanoma cutaneous malignant 3 antibody; MGC14458 antibody; p34 cdk4 antibody; PSK J3 antibody; PSK-J3 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P11802 Immunogen:Peptide sequence around aa.295~299(L-H-K-D-E)derived from CDK4. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC; WB:1:500-1:1000, IHC:1:50-1:200 Background: Cyclin-dependent kinase activity is regµLated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellµLar mitogenic stimµLi for the release and degradation of p27, which affects progression throµgh the restriction point and pRb-dependent entry into S-phase The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression In HeLa cells, upon UV irradiation, upregµLation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression throµgh the G2-phase of the cell cycle Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C.J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CDK4 Research Areas:Isotype/Loading Controls;Epigenetics and Nuclear Signaling;Cancer;Cell biology