ELISA Canine C-C motif chemokine 26 (CCL26)

Reactivity:Canine (Canis familiaris; Dog) UniProt:Q64H35 Abbreviation:CCL26 Alternative Names:IMAC; MGC126714; MIP-4a; MIP-4alpha; SCYA26; TSC-1; CC chemokine IMAC|chemokine N1|eotaxin-3|macrophage inflammatory protein 4-alpha|small inducible cytokine A26|small inducible cytokine subfamily A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.5% Inter-AssayCV:?6.3% Recovery:0.91 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate CCL26 in samples. An antibody specific for CCL26 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL26 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for CCL26 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL26 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Chemokine (C-C motif) ligand 26 (CCL26) is a small cytokine belonging to the CC chemokine family that is also called Eotaxin-3, Macrophage inflammatory protein 4-alpha (MIP-4-alpha), Thymic stroma chemokine-1 (TSC-1), and IMAC. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimµLated with the cytokine interleukin 4.CCL26 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. This gene for chemokine is located on chromosome 7. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).