ELISA Bovine Insulin-like peptide INSL6 (INSL6)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q32L79 Abbreviation:INSL6 Alternative Names:RIF1; insµLin-like peptide 5|relaxin/insµLin-like factor 1 Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.2 pg/mL Intra-AssayCV:?4.5% Inter-AssayCV:?7.3% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate INSL6 in samples. An antibody specific for INSL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyINSL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for INSL6 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL6 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:INSL6 contains a classical signature of the insµLin superfamily and is significantly similar to relaxin and relaxin-like factor. This gene is preferentially expressed in testis. Its expression in testis is restricted to interstitial cells surrounding seminiferous tubµLes, which sµggests a role in sperm development and fertilization. The deduced 213-amino acid protein, which shares 45% sequence identity with the mouse protein, contains the classic B-C-A domain configuration, including the 6 cysteines in the B and A domains, of the insµLin/relaxin family proteins. Northern blot analysis revealed expression of a 1.2-kb transcript confined to testis. Immunohistochemical analysis demonstrated expression restricted to interstitial cells in mouse testis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).