ELISA Canine Deleted In Liver Cancer 1 (DLC1)

Reactivity:Canine (Canis familiaris; Dog) UniProt:B9VTT2 Abbreviation:DLC1 Alternative Names:ARHGAP7; FLJ21120; HP; STARD12; p122-RhoGAP; Rho-GTPase-activating protein 7|StAR-related lipid transfer (START) domain containing 12|deleted in liver cancer 1 variant 2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.2% Inter-AssayCV:?9.3% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLC1 in samples. An antibody specific for DLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Deleted in Liver Cancer 1 is deleted in the primary tumor of hepatocellµLar carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is sµggested that this gene is a candidate tumor suppressor gene for liver cancer, as well as for prostate, lung, colorectal, and breast cancers.DLC1 is frequently inactivated in hepatocellµLar carcinoma, as well as some nasopharyngeal, lung, breast, prostate, kidney, colon, uterine, ovarian, and gastric cancers. The DLC1 protein contains four major functional domains: an N-terminal sterile ? motif (SAM), a serine-rich (SR) region, a Rho-GAP domain, and a C-terminal steroidogenic acute regµLatory protein related lipid-transfer (START) domain. DLC1 is localized to focal adhesions located at the periphery of cells. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).