ELISA Bovine Inactive serine protease 35 (PRSS35)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q5E9X7 Abbreviation:PRSS35 Alternative Names:C6orf158; MGC46520; dJ223E3.1; inactive serine protease 35 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.4% Inter-AssayCV:?8.3% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRSS35 in samples. An antibody specific for PRSS35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRSS35 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS35 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Proteolytic degradation of extracellµLar matrix components has been sµggested to play an essential role for the occurrence of ovµLation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovµLation, are not required in this process. PRSS35, which was upregµLated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovµLation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granµLosa cells of preovµLatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovµLation and CL formation and regression, and that PRSS23 may play a role in follicµLar atresia. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).